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Mass Spec Instruments

The Chemistry Department Mass Spectrometry Facility is equipped with six state-of-the-art instruments.

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Q-Tof Ultima API, Micromass UK Limited

Ionization: Electrospray ionization (ESI, ±ve), Atmospheric pressure chemical ionization (APCI, ±ve)

Mass range: m/z 50-32,000 (M.W. up to 100 KDa)

Resolution: 15,000 FWHM / < 10 PPM mass accuracy

Inlet system: Water 2695 separation module

Analytes: Organic and bio-organic molecules / Polar and non-polar compounds

Software: Masslynx 4.1


Q-Exactive, Thermo Scientific

 

Ionization: ESI Pos & Neg, APCI Pos & Neg and Atmospheric Pressure Photo ionization (APPI, Pos & Neg)

Mass range: m/z 50-6000 (M.wt up to 80KDa of protein/peptide)

Resolution: 140,000 FWHM @ m/z 200 / < 1 ppm error (Internal calibration) and < 3 ppm (External calib)

Inlet System: Dionex Ultimate 3000 (RSLC)

Analytes: Organic and Bio-organic compounds/Polar and Non polar compounds, Proteomic and Metabolomic samples.

Software: Xcaliber 2.1, Proteome Discoverer 1.3, Mass Frontier 7.0, MetWorks 1.3sp1, Seive 2.0 and Protein Deconvolution 3.0  


LCMS-2020, Shimadzu Instrument

Ionization: Electrospray ionization (ESI, ±ve),  Atmospheric pressure chemical ionization (APCI, ±ve)

Mass range: m/z 10-2000 (M.W. up to 40,000)

Inlet system: Shimadzu Ultra Fast Liquid Chromatography (UFLC) 

Analytes: Organic and bio-organic molecules/Polar and non-polar compounds

Software: Labsolution   

PDF: LCMS Quick Guide


GCT, Micromass UK Ltd

Ionization: Electron ionization (EI, ±ve)

Mass range: m/z 10-600

Resolution: 7000 FWHM / < 15 ppm Mass Accuracy

Analytes: Volatile organic or inorganic compounds/Non-polar compounds with manual probe

 


GCMS-QP2010S, Shimadzu Instrument

 

Ionization: Electron ionization (EI, +ve)

Mass range: m/z 10-900

Inlet system: Shimadzu Gas Chromatography (GC)

Analytes: Volatile organic or inorganic compounds/non-polar compounds

PDF: GCMS Quick Guide


MALDI, Voyager-DE PRO Instrument

Ionization: Desorption ionization (±ve)

Mass range: Up to 300KDa

Resolution: 2500 (linear mode), 10,000 (reflector mode) FWHM

Analytes: High molecular weight compounds (peptides, proteins, polymers etc) / Polar and non-polar compounds   

PDF: MALDI Quick Guide